![]() Biocontrol bacteria have a significant promoting effect on colonization in the environment surrounding plants. These biofilms are described as microcolonies, aggregates, cell clusters, etc. In the process of colonizing host plants and bacteria, bacteria can form biofilms on their surfaces. Later, in research of the interaction between plants and bacteria, researchers found that biofilms are particularly important for the colonization of bacteria, and biofilms have since become a research hotspot in related fields (Yang et al. In 1995, Costerton first proposed the concept of a biofilm (Costerton et al. There is evidence that the formation of a biofilm is an important step in the sustainable development and spread of bacteria (Guilhen 2017), including the exchange of intermediate genes and their synergy (Davey and O’Toole 2000). Statistically, most bacteria in nature exist in biofilms (Whitchurch et al. The resistance of bacteria in this state is much stronger than that in the planktonic state, and bacteria in this state have the advantages of sensing the surrounding environment and rapidly ingesting nutrients (Song et al. This is the first report of JK-SH007 producing a long-lasting biofilm that guarantees antagonism.īacterial biofilms are organized and distinct three-dimensional structures of life groups (Mah et al. These results indicate the importance of adding nutrients to JK-SH007 biocides produced on a commercial scale. ![]() The possible mechanism by which JK-SH007 was inhibited by biofilm formation under natural culture was revealed. RT-qPCR experiments showed that an increase in the expression of the suhB gene promoted JK-SH007 biofilm formation, while an increase in the expression level of the ropN gene inhibited JK-SH007 biofilm formation. The optimal medium for biofilm formation was TSB medium, 1% glycerol and 50 mM MgSO 4. The optimized system showed that the optimal incubation time for JK-SH007 biofilm formation was 14 h, the optimal temperature of the static culture was 25 ☌, and the optimal pH was 5. Therefore, we constructed and optimized a biofilm-forming system to produce a large number of stable JK-SH007 biofilms. The addition of glycerol and MgSO 4 to TSB medium stimulated biofilm production, increased the resistance of JK-SH007 to disease, enhanced the survival of JK-SH007 in nutrient-poor environments and maintained the antagonistic ability of JK-SH007 against the poplar canker pathogen. To explore the mechanism of action of biofilms in the biocontrol process, we manipulated various exogenous factors to explore the morphology of the JK-SH007 biofilm in vitro. ![]() Bacterial biofilms can ensure the long-term colonization of a host. Its powerful biocontrol function is inseparable from its successful colonization of poplar trees. Burkholderia pyrrocinia JK-SH007 is an important biocontrol strain for the prevention and treatment of poplar canker disease. ![]()
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